The mean colloid volume of the dolphin thyroid gland and human thyroid gland was 1.22×10 5 µm 3 and 7.02×10 5 µm 3 respectively. The distribution of large and small follicles within the thyroid gland was also found to be random in both the dolphin and human thyroid gland however, the size of follicles appeared to decrease as a function of increasing age in the dolphin thyroid gland. In both dolphin and human thyroid glands, the size of the follicles tended to be quite variable. The present study determined the colloid volume using simple stereological principle and the nuclear-to-cytoplasmic ratio of 4 Indo-Pacific bottlenose dolphins and 2 human thyroid glands. Direct measurements of colloid volume and nuclear-to-cytoplasmic ratio of the follicular cells may provide important information about thyroid gland function such as hormone storage and secretion, which helps understand the changes at morphological and functional levels. Volume estimation is also important in stereological studies. Determining thyroid volume is important in assessing growth and involution. The mammalian thyroid gland maintains basal metabolism in tissues for optimal function.
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